書誌事項
- タイトル別名
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- Development of an endotoxin-specific Limulus amebocyte lysate test blocking .BETA.-glucan-mediated pathway by carboxymethylated curdlan and its application.
- ダイカジョウ ノ カルボキシメチルカ カードラン ニ ヨル G インシケイ ソ
この論文をさがす
説明
We developed a simple new endotoxin-specific assay method that uses Limulus amebocyte lysate (LAL) containing a sufficient amount of a water-soluble (1→3)-β-D-glucan derivative as a blocker of the (1→3)-β-D-glucan-mediated coagulation pathway. The addition of 0.1mg/ml or more of carboxymethylated (1→3)-β-D-glucan completely blocked the activation of LAL by (1→3)-β-D-glucan itself. The assay of endotoxin was unaffected by the presence of 1mg/ml carboxymethylated (1→3)-β-D-glucan. Spiked endotoxin was recovered well from β-glucans by the turbidimetric kinetic method with LAL containing 1mg/ml of carboxymethylated (1→3)-β-D-glucan. Besides, this new LAL formulation was applied for an endotoxin-specific assay by the conventional gel-clot method or the chromogenic method. Gram-negative bacteria were specifically detected by the turbidimetric kinetic method with the LAL formulation. This LAL formulation may be used for an endotoxin-specific assay not only in pharmacology but also in clinical microbiology.
収録刊行物
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- 日本細菌学雑誌
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日本細菌学雑誌 45 (6), 903-911, 1990
日本細菌学会
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詳細情報 詳細情報について
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- CRID
- 1390282681425695232
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- NII論文ID
- 130001386528
- 10010734304
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- NII書誌ID
- AN00189800
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- COI
- 1:CAS:528:DyaK3MXktVals78%3D
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- ISSN
- 18824110
- 00214930
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- NDL書誌ID
- 3696666
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- PubMed
- 2082026
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
