Anaerobic Culture Method with Steel Wool

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  • Steel woolを用いる嫌気性培養法
  • Steel wool オ モチイル ケンキセイ バイヨウホウ

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Abstract

Good results were obtained in anaerobic culture of Clostridium and others by using steel wool, which was now available in Japan, as a substitute for iron wool used in Parker's anaerobic culture.<BR>In order to obtain anaerobiosis rapidly, the optimal concentration of CuSO4 7H2O, that of Tween 80, grade of steel wool and so on were examined. Two jars (3 and 4 liters, respectively) with cock were used and anaerobiosis in these jars was measured by hand-made open legged manometer at about one hour intervals.<BR>The most effective procedure is as follows: Ten grams of steel wool of grade O per 3 liters of jar volume is soaked into 500ml of 1.2% CuSO4 7H2O solution, which also includes 0.08% Tween 80 or sugar ester and is adjusted to pH 1.5 with conc. H2SO4, till the decoloration of the solution results in (for 0.5 to 1 minute). Then steel wool is transferred into the far containing alkaline-methylen blue indicator in a bottle and plates inoculated. At this time, small volume of H2 gas is produced, so that removal of air equivalent to it is preferable before sealing of jar.<BR>By the above established method, good growth of many strains belonging to pathogenic Clostridium, L. bifidus, and V. alcalescens was attained. However, as to isolation of ruminal bacteria, the result by this method is inferior to that of Hungates rolled tube method.

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