Infection and Development of Nosema mesnili NM-HC-A8801(Microsporida: Protozoa) in Lepidopteran Cell Line.

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  • YOKOTA Satoko
    Institute of Biological Control, Faculty of Agriculture, Kyushu University
  • YASUNAGA Chisa
    Institute of Biological Control, Faculty of Agriculture, Kyushu University
  • KAWARABATA Takeshi
    Institute of Biological Control, Faculty of Agriculture, Kyushu University
  • HAYASAKA Syoji
    National Institute of Sericultural and Entomological Science
  • TSUDA Katsuo
    Institute of Agro-Environmental Science, Fukuoka Agricultural Research Center

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Other Title
  • 鱗し目昆虫病原性微胞子虫Ｎｏｓｅｍａ　ｍｅｓｎｉｌｉＮＭ‐ＨＣ‐Ａ８８０１株のＡｎｔｈｅｒａｅａ　ｅｕｃａｌｙｐｔｉ細胞系への接種の増殖
  • 鱗翅目昆虫病原性微胞子虫Nosema mesnili NM-HC-A8801株のAntheraea eucalypti細胞系への接種と増殖
  • リンシモク コンチュウ ビョウゲンセイ ビホウシチュウ Nosema mesn

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Abstract

Spores of Nosema mesnili NM-HC-A8801 primed with 0.1-N KOH solution were inoculated in Antheraea eucalypti cell cultures at a ratio of 30 spores per cell. The inoculated insect cells were cultured at 27°C in GRACE'S medium supplemented with 10% fetal bovine serum. At 1h post-inoculation, the percentage of hatched spores was approximately 20%, and about 5% of A. eucalypti cells were infected initially with sporoplasms. At 96h post-inoculation, many-coiled mature spores were produced and an infected A. eucalypti cell contained about 40 protozoans. A rapid increase in cell infection rates was observed and the percentage of infected cells reached 90% 120h post-inoculation. In the life cycle, N. mesnili NM-HC-A8801 was diplokaryotic and showed disporous development which are typical of the genus Nosema. However, the spread of infection and spore production were markedly different from N. bombycis and Nosema sp. NIS M 11.

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