?EＧｅｏｔｒｉｃｈｕｍ　ｃａｎｄｉｄｕｍ?Fの酵母相および菌糸相細胞ならびに細胞壁組成の比較

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  • Comparison of Cell Body and Cell Wall Compositions between the Yeast-like and Mycelial Phases of <i>Geotrichum candidum</i>
  • Geotrichum candidum ノ コウボソウ オヨビ キンシソウ サ

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Cells of yeast-like (Y) and mycelial (M) phases were obtained from cultures of Geotrichum candidum on basal media containing 1% glucose and 0.5% glycerol, respectively. Their cell walls were fractionated by successive treatment with sonic vibration and centrifugation.<br>The Y-phase cells contained slightly larger amounts of protein, RNA and phosphorus (12.0%, 1.9%, and 0.8%) than the M-phase cells (9.0%, 1.2%, and 0.6%), whereas the hexosamine content of the M-cells (9.7%) was slightly greater than that of the Y-cells (8.0%).<br>There was no significant difference between the two phases in the amount of total sugar (Y: 57%, M: 60%), acid-or alkali-soluble polysaccharides, or lipids (Y: 6%, M: 5%). The amounts of galactose and mannose in the supernatant obtained after sonic vibration were found larger in the M-phase cells than in the Y-phase cells. The ratio of the amount of galactose plus mannose in the supernatant to that of glucose in the cell wall was approximately five percent of the molar ratio.<br>The wall lipid of the M-cells extracted with CHCl3-MeOH contained fatty acids, such as C14, C15, C16, C16:1, C17, C17:1, C18, C18:1, C18:2 and C19:1, at a molar ratio of 1:2:17:2:2:3:4:38:30:1. Therefore, the cell wall contained three times as much unsaturated fatty acids as saturated fatty acids.<br>Amino acid analyses of the wall protein of cells of both phases revealed that the Y-cells had a little more aspartic acid, glutamic acid and glycine (9.0%, 9.7%, and 8.1%) than the M-cells (8.2%, 8.5%, and 5.9%), and that the M-cells had a little more alanine (12.8%) than the Y-cells (9.8%).<br>These results suggest that there may be no qualitative difference in the chemical composition of the cell wall between the cells of both phases, although small differences may be seen in the contents of several components and/or the composition between these cells.

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