Microautoradiographic Studies on the Metabolism of Protein and Ribonucleic Acids in Posterior Silkglands

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  • MIURA Yoshiaki
    Department of Biochemistry, Division of Health Care and Nursing, Faculty of Medicine, University of Tokyo
  • ITO Hirowo
    Department of Biochemistry, Division of Health Care and Nursing, Faculty of Medicine, University of Tokyo
  • SHIGEMATSU Akiyo
    Department of Biochemistry, Division of Health Care and Nursing, Faculty of Medicine, University of Tokyo

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Other Title
  • ミクロオートラジオグラフによる絹糸せんの核酸およびタンパク代謝の研究
  • ミクロオート ラジオ グラフ ニ ヨル キヌイト セン ノ カクサン オヨビ タンパク タイシャ ノ ケンキュウ

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1. A semiquantitative microautoradiographic technic was applied to the metabolic studies of protein as well as ribonucleic acids in the posterior silkglands during the fifth instar period of Bombyx mori.<br>2. Actually no incorporation of 32P or 14C-glycine into deoxyribonucleic acid was observed. Whereas, a remarkable renewal of RNA-32P was shown around 2nd and 3rd days of the fifth instar. Later the incorporation of 14C-glycine into fibroin began from the 3rd day and reached to a maximum at the 7th day. These observations led to the conclusion that the co-synthesis of ribonucleic acid during the fibroin biosynthesis is not required.<br>3. Different sites of the incorporation of ribonucleic acid were observed in the different labeled compounds: 32P was incorporated rather in external area of silkglands, whereas 14C-glycine was incorporated solely into the nuclear RNA. Both labeled compounds did not enter into the RNA-core after RN-ase treatment.

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