家蚕の角皮に関する研究

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タイトル別名
  • Studies on the Cuticle of the Silkworm, <i>Bombyx mori</i> L.
  • カサン ノ カク ヒ ニ カンスル ケンキュウ 12
  • XII. Pore Canals and Epicuticle in the Larval Cuticle
  • XII. 幼虫角皮における孔管と表角皮の観察

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抄録

Many authors in Japan have thrown doubt upon the existence of pore canal in the silkworm larvae, because it was invisible in ordinary paraffin sections. However, when the fresh sections cut with freezing microtome are examined in water under a light microscope, pore canals are clearly seen penetrating through both the exo- and the endocuticular layers of the larvae. Arising directly from the epidermal cells all the canals (2∼4μ in diameter) run a parallel vertical course in deeper part of the cuticle, but near their middle part they branch off towards the surface (with an average diameter of 1∼2μ in a single branch). At the outer extremities the canals seem to reach beneath the epicuticle of each tubercle of the integument.<br>The epicuticle of the silkworm is not readily distinguished from its underlying exocuticle from the staining properties of the paraffin section examined by means of the light microscope. But both layers can be separated from one another, if the cuticle is examined under the phase-contrast microscope either in the freezing or in the paraffin sections even if they are not stained.

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