ＰＣＭＢ処理巨大菌芽胞の発芽性におよぼす種々薬品処理の影響

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  • The Effect of Various Extraction Procedures on Germination of PCMB-treated <i>Bacillus megaterium</i> Spores
  • PCMB ショリ Bacillus megaterium ガホウ ノ ハツガセ

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When treated with p-chloromercuribenzoate (PCMB), spores of Bacillus megaterium ATCC 19213 were changed to be able to germinate with glucose. When further treated with 8M urea, the PCMB-treated spores lost their ability to germinate with glucose. The ratio of germination reaction to alanine-inosine solution remained high after the two treatments. In the urea treatment, 50% of the mercury contained was released from the PCMB-treated spores.<br>The urea extract of the PCMB-treated spores exhibited at least two fractions of mercury by Sephadex G-25 column chromatography. The greater portion of the mercury contained was found in one fraction consisting of low molecular compounds which were ninhydrin-positive, and the remaining portion in the other fraction consisting of high molecular compounds. These results suggest that the PCMB-bound components eluted in the extract may play an important role in a specific reaction of spores against germinants.

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