Establishment of monoclonal antibody mNI-11 which induces homotypic cell aggregation of LPS-treated U937 cells.

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  • ＬＰＳ処理単球系細胞株（Ｕ９３７）のｈｏｍｏｔｙｐｉｃ　ｃｅｌｌ　ａｇｇｒｅｇａｔｉｏｎを誘導するモノクローナル抗体
  • LPS ショリ タンキュウケイ サイボウカブ U937 ノ homotypic

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Abstract

A mouse monoclonal antibody (mAb), designated as mNI-11, was produced. The reactivity of mNI-11 was assessed by immunofluorescence assay with various cells. Myelomonocytic cell line, U937, and lipopolysaccharide (LPS)-treated U937 (LPS-U 937 cells) were found to bind strongly to the mAb. EBV-B cell line and peripheral blood mononuclear cells (PBMCs) were found to bind moderately to it. This mAb strongly induced homotypic cell aggregation of LPS-U937 cells. The mAbs to CD 18 and CD 54 completely inhibited the LPS-U 937 cell aggregation induced by mNI-11. The surface antigens of the U 937 and LPS-U 937 cells recognized by mNI-11 had a molecular size of 95-97kDa as determined by immunoblotting analysis.

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