イネ黄萎病ウイルス保毒ツマグロヨコバイの識別法について

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  • Detecting Method of the Viruliferous Green Rice Leafhopper, <i>Nephotettix cincticeps</i> UHLER, a Vector of the Yellow Dwarf Disease of the Rice Plant
  • イネ オウイビョウ ウィルスホドク ツマグロヨコバイ ノ シキベツホウ ニ ツイテ

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Because of taking long incubation period, the method of virus inoculation for the plant by means of insect which has been often applied to discriminate a viruliferous insect is not considered practical.<br>The adipose tissues of abdomens of the insects were pressed out on a slide glass and a drop of nile blue saline solution (0.5per cent dye in 0.9per cent saline) was added to it. In the healthy individuals, the whole adipose tissues are stained deeply blue with nile blue solution, while in the affected ones many globules of various sizes, stained red with nile blue solution, are observed in the blue stained cytoplasm.<br>As reported in the previous paper (TAKAHASHI & SEKIYA, 1962), the fact that the adipose cells of the viruliferous insects are conspicuously reticulated in the paraffin preparations, may be due to dissolving of the lipids by various solvents such as alcohol, tolol and xylol which are used during the course of the preparation.<br>In the examinations of fatty tissues by the paraffin section method, 90per cent of individuals show characteristic reticular structure in the viruliferous leafhoppers, with exception of 10per cent in which the cytoplasm seems to be normal. In the nonviruliferous group, however, the cytoplasm is generally normal, i.e., 86per cent of individuals are homogeneous in structure and 14per cent are reticular. Similar results are obtained from the observation by means of the simple procedure mentioned above. Namely the red globules are observed in insects of 82per cent from the affected group, and of 25per cent from the non-affected ones.<br>The new method was applied in the actual survey of the insect vectors in several localities of Nagano Prefecture where the virus disease was highly prevailing, and good results were obtained in general. The data from this method coincide with ones from the standard section method as well as the direct transmission method.

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