生体染色ならびに組織学的観察による油蚕性細胞と正常細胞における色素の吸着性の比較

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  • Comparison in Adsorption of Dyes between Translucent and Normal Silkworm-Cells by Vital Staining and Histological Observations
  • セイタイ センショク ナラビニ ソシキガクテキ カンサツ ニ ヨル アブラ カイコセイ サイボウ ト セイジョウ サイボウ ニ オケル シキソ ノ キュウチャクセイ ノ ヒカク

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The author performed histochemical as well as histological observations on the vitally stained and fixed materials using mottled translucent silkworm larvae, translucent and their normal segregants with many dyes. The results obtained were as follows: <br>1. In the case of injection and oral administration of several basic dyes to the mottled larvae, the opaque (normal) portion in the integument was stained deeply but translucent portion was scarcely colored, and silk glands and Malpighian tubes were stained in mosaic feature. Especially neutral red showed excellent stainability in those tissues. While the integument scarcely colored after injection and oral administration of acidic dyes, but many other organs were stained uniformly with acidic dyes.<br>2. There was no great difference between nucleic acid of normal cells and that of translucent ones by pyronine-methyl green staining.<br>3. As to fixed materials, epidermal cells, silk glands and Malpighian tubes of both translucent and normal larvae were stainable with neutral red and nile blue, translucent cells of the epidermis in mottled mutant larva being stained more deeply than normal cells. The above mentioned result is different from that of vital staining with basic dyes.<br>4. It was observed that there were many vacuoles in the epidermal cell of translucent larva and of the translucent portion of mottled larva, but normal epidermal cells had no or few vacuoles, the epidermal cytoplasm of the normal larva being more compact than that of the translucent one.<br>5. No clear difference was observed between translucent and normal segregants concerning phosphatase activity.<br>6. In view of the above mentioned results, it is conceivable that the electric charge and properties of cytoplasm play an important role for adsorption and reservation of uric acid and some pigments and dyes in epidermal cells.

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