ハリガネムシに関する研究

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タイトル別名
  • Researches on the Wireworm, <i>Melanotus caudex</i> LEWIS
  • ハリガネムシ ニ カンスル ケンキュウ 14
  • XIV. On the Bacteria Living in the Body of Wireworm
  • 第14報 マルクビクシコメツキ幼虫の体内細菌

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1. Here, the bacterial flora in the body of wireworms was studied.<br>2. Body surface of the worm, sterilized with alcohol and corrosive sublimate solution, was washed with sterile water for several times. The body, crushed and emulsified in a test tube containing 10cc of sterile water, was diluted to concentrations of 10-2, 10-3 and 10-4, 1cc of which was taken and was cultured with beef extract agar and potato agar at 25°C. Colonies on the plate culture were examined 24∼48hr after the treatment.<br>3. Eight organisms (four genera) belonging to Eubacteriineae were isolated. The organisms S1, S4 and S6, were always isolated from wireworm, regardless of the collecting dates and places. S4 was isolated more in number than S6 and S1 at any time. The other organisms were found very few in the worm's body at any time, but they increased to some extent at their active (feeding) period. Therefore it seems probable that those organisms were taken into the body together with worm's food.<br>4. When the wireworm was bred in the sterilized breeding bottle in laboratory, S1, S4 and S6 were found while the others isolated disappeared about 10 days after breeding. S4 increased in number rapidly at the 30th day after breeding, while S1 and S6 did not increase.<br>5. S4 and S6, though not isolated from body fluid of wireworm, were much isolated from fat body. S4 was always more in number than S6.<br>6. In S4, the minimum temperature for growth was between 10°C and 15°C; this almost coincides with the average soil temperature (11∼13.5°C) at 5cm under soil surface during the period fromm the last ten days of March to the first ten days of April, when the wireworm begins to migrate near to the soil surface. It seems that the optimum temperature for growth lies between 30°C and 40°C.<br>7. Using the modified STAR'S medium, the response of S4 to cotton seed oil was examined.BTB turned to yellow. This shows that S4 resolved the cotton seed oil into fatty acid and glycerin. It may therefore be assumed that S4 resolves the body fat of worm.<br>8. S4 found in the worm killed with insecticides was greater in number than that of the normal individual.

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