数種りん翅目昆虫の中腸細胞質で増殖する Enterobacter sp. (2) : 血清学的性状について

DOI 日本農学文献記事索引 Web Site

書誌事項

タイトル別名
  • Study on the serological reaction of Enterobacter sp. Multiplying in the cytoplasm of midgut cells of some lepidopterous larvae.
  • 数種りんし目昆虫の中腸細胞質で増殖するＥｎｔｅｒｏｂａｃｔｅｒ　ｓｐ．　ＩＩ　　血清学的性状について
  • スウシュリン シモク コンチュウ ノ チュウチョウ サイボウシツ デ ゾウショ
  • II. 血清学的性状について

この論文をさがす

抄録

The isolate (Enterobacter sp.) from the infected larvae was analysed. By the percoll density gradient method, bacterial cells obtained as a white opaque band in the center of the centrifugation tube were collected. The purified bacteria were injected into rabbits as an antigen to prepare an antiserum. The antiserum reacted only with the bacterial cells of this particular isolate when the electrosyneresis and fluorescent antibody method were applied. No reaction was observed with other bacteria used as controls. A serum separately prepared against Serratia marcescens was found to react only with Serratia marcescens, without showing any reaction with the cells from Enterobacter sp. Subsequent observation by the electrosyneresis method revealed that the inoculation of the bacterial cells into larvae led to the detection of the specific antigen only in the infected larvae. Therefore it was possible to determine by the serological reaction whether the infection had taken place. The multiplication of the bacteria in the cytoplasm of the midgut was also observed with the fluorescent antibody method. Specific fluorescence was observed only in the cytoplasm of the cylindrical cells in the midgut. This reaction was intensified with the multiplication of the bacteria. The growth phase observed with an optical-microscope was identical with that observed with an electron-microscope.

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ