書誌事項
- タイトル別名
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- Detection of two Shiga-like toxins from Escherichia coli O157:H7 isolates by the polymerase chain reaction method.
- イデンシ ゾウフクホウ PCR ニ ヨル シガ セキリキンヨウ ドクソ イデン
この論文をさがす
説明
Fourteen isolates of E. coli O157:H7 and five isolates of S. dysenteriae type-1 were examined by polymerase chain reaction (PCR) for the structural genes (slt-I or slt-II), encoding Shiga-like toxins (SLTs). The two primer pairs (V1; 5'AGTTAATGTGGTGGCGAA and V2; 5'GACTGCGTCAGTGAGGTT for SLT-I, V3; 5'TTCGGTATCCTATTCCCG and V4; 5'TCT CTGGTCATTGTATTA for SLT-II) used were of the same positions representing the DNA sequence covering 471bp of the slt-I or slt-II. A 5-μl portion of boiled bacterial culture broth was used as template DNA in a PCR-reaction mixture of 50μl.<br>Two classes, slt-I alone or both slt-I and slt-II, were recognized in E. coli strains. All of S. dysenteriae type-1 strains examined contained slt-I alone. Our results indicate that PCR using these primer pairs is a simple, rapid, sensitive, and specific method and suitable for use in routine diagnostic microbiology laboratories.
収録刊行物
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- 日本細菌学雑誌
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日本細菌学雑誌 45 (3), 649-652, 1990
日本細菌学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390282681427102208
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- NII論文ID
- 130001386441
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- NII書誌ID
- AN00189800
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- COI
- 1:CAS:528:DyaK3MXhtlOnurY%3D
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- ISSN
- 18824110
- 00214930
- http://id.crossref.org/issn/00214930
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- NDL書誌ID
- 3671559
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- PubMed
- 2205737
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
