イセリヤカイガラムシの脂質成分へ(U-14C)酢酸ナトリウムのとりこみ

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タイトル別名
  • イセリヤカイガラムシの脂質成分への〔Ｕ‐?Q１４?RＣ〕酢酸ナトリウムの取り込み
  • Incorporation of [U-<sup>14</sup>C] Acetate into the Lipids of Scale Insect, <i>Icerya purchasi</i> MASKELL (Hemiptera: Margarodidae)
  • イセリヤカイガラムシ ノ シシツ セイブン エ U 14C サクサン ナトリウ

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Incorporation of [U-14C] acetate into the lipids of scale insect Icerya purchasi was investigated. The insects were placed on a Parafilm® membrane over a liquid food containing the labelled compound and allowed to suck up the liquid through the stylet piercing the membrane in the dark. The marker was incrorporated into individual lipid classes, and after 1 hour of feeding the relative radioactivities of the classes were as follows: phospholipids>(S)-3-acetyl-1, 2-diacylglycerols (ADG)>free fatty acids≈diacylglycerols>normal triacylglycerols (n-TG)>monoacylglycerols. The ADG was fractionated into acetyl, diacyl and glyceryl moieties to determine the distribution of radioactivity within ADG. The acetyl moiety from the 3-position of sn-glycerol contained a higher percentage of radioactivity than any of other moieties. However, by feeding for 3 to 12 hours, the radioactivity was higher in the diacyl and glyceryl moieties, with marked decrease in the acetyl moiety. These results suggest that ADG is a carrier of the acetyl group and a link in the formation of n-TG. Analysis of fatty acids in the lipids showed that the marker was also incorporated into the di-unsaturated acid fraction, which consisted almost entirely of linoleic acid suggesting the de novo synthesis of this fatty acid by the insect. In the experiment using ovipositing insects, the marker was found to be incorporated into the lipids in both the eggs and egg sacs, and also into the mother bodies indicating that the insects continue feeding even during the preiod of oviposition.

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