A Simple method for differentiation of hydrogen sulfide-producing bacteria by the pH-dependent EDTA-sensitivity test.

DOI Web Site PubMed 2 Citations
  • KIDA Nori
    Center for Inspection of Imported Foods and Infectious Diseases, Yokohama Quarantine Station, Ministry of Health and Welfare
  • MATSUZAKI Mitsuhiro
    Center for Inspection of Imported Foods and Infectious Diseases, Yokohama Quarantine Station, Ministry of Health and Welfare
  • MATSUKAWA Akihiro
    Center for Inspection of Imported Foods and Infectious Diseases, Yokohama Quarantine Station, Ministry of Health and Welfare
  • SUZUKI Sosuke
    Center for Inspection of Imported Foods and Infectious Diseases, Yokohama Quarantine Station, Ministry of Health and Welfare
  • USHIAMA Nobuyuki
    Center for Inspection of Imported Foods and Infectious Diseases, Yokohama Quarantine Station, Ministry of Health and Welfare
  • KAWAHARA Kazuyoshi
    Department of Bacteriology, Center for Basic Research, the Kitasato Institute
  • TAGUCHI Fumiaki
    Department of Microbiology, School of Hygienic Sciences, Kitasato University

Bibliographic Information

Other Title
  • ｐＨ依存ＥＤＴＡ感受性試験による硫化水素産生菌の簡便な鑑別法について
  • pH イゾン EDTA カンジュセイ シケン ニ ヨル リュウカ スイソサン

Search this article

Abstract

The pH-dependent EDTA-sensitivity test was performed to differentiate several strains of bacteria forming black colonies by the production of hydrogen sulfide on TCBS (thiosulfatecitrate-bile salt-sucrose agar) medium (tentatively designated as hydrogen-sulfide production bacteria).<br>Two halotorelant strains of 16 hydrogen sulfide-producing strains showed the same bacteriological properties and isoprenoid quinone type as did a reference strain of Proteus mirabilis and were classified into the EDTA-insensitive group as were P. mirabilis and P. vulgaris.<br>On the other hand, the other 14 halophilic strains, showing similar but not identical bacteriological properties or the isoprenoid quinone type to those of Shewanella putrefaciens IFO 3908, were classified into the “EDTA-sensitive (at pH 5)” group as were some species of the genus Vibrio. By the same sensitivity test, S. putrefaciens IFO 3908 was classified into the “EDTA-sensitive (at any pH)” group.<br>These results indicate that the pH-dependent EDTA-sensitivity test is useful for differentiation of bacterial isolates producing hydrogen sulfide and having similar bacteriological properties.

Journal

Citations (2)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top