数種鱗翅目昆虫培養細胞系における微胞子虫Nosema mesnili NM-HC-A8801株の感染・増殖および持続感染系の樹立
書誌事項
- タイトル別名
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- Continuous Culture of Nosema mesnili NM-HC-A8801 (Microsporida: Nosematidae) in Four Lepidopteran Cell Lines.
- 数種鱗し目昆虫培養細胞系における微胞子虫Ｎｏｓｅｍａ ｍｅｓｎｉｌｉ ＮＭ‐ＨＣ‐Ａ８８０１株の感染・増殖および持続感染系の樹立
- スウ シュリン シモク コンチュウ バイヨウ サイボウケイ ニ オケル ビホウ
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Spores of Nosema mesnili NM-HC-A8801 primed with 0.1N KOH solution were inoculated into four lepidopteran cell lines (Antheraea eucalypti, Spodoptera frugiperda SF21AEII, Bombyx mori BmN-4, and Trichoplusia ni hi5), and the microsporidian growth and spread of infection were investigated. At a ratio of 30 spores per cell, 3% to 5% of host cells were infected initially with sporoplasms at 1h post-inoculation. The number of the parasites in an infected cell started to increase at 24h post-inoculation, depending on the merogony of N. mesnili NM-HC-A8801. At 48h post-inoculation, when secondary infective forms appeared, rapid increases were observed in the number of parasitized cells. The percentage of infected cells at 120h post-inoculation in the A. eucalypti, S. frugiperda SF21AEII, and B. mori BmN-4 cell lines reached approximately 90%, 60%, and 20%, respectively. Persistent infections of N. mesnili NM-HC-A8801 could be maintained through several passages of these cell cultures. In particular, the A. eucalypti and S. frugiperda SF21AEII cell lines showed high compatibility with this microsporidium. On the other hand, only 4% of T. ni hi5 cells were infected at 120h post-inoculation, and infected cells disappeared after the second passage.
収録刊行物
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- 日本応用動物昆虫学会誌
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日本応用動物昆虫学会誌 41 (3), 147-152, 1997
日本応用動物昆虫学会
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詳細情報 詳細情報について
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- CRID
- 1390282681427342720
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- NII論文ID
- 110001113632
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- NII書誌ID
- AN00186121
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- ISSN
- 13476068
- 00214914
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- NDL書誌ID
- 4285662
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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