蚕体組織のウレアーゼ活性に及ぼす飼料の影響

DOI 日本農学文献記事索引 Web Site 被引用文献2件

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タイトル別名
  • Effects of Diets on Urease Acitivities in Different Tissues of the Silkworm, <i>Bombyx mori</i> (Lepidoptera: Bombycidae)
  • サンタイソシキ ノ ウレアーゼ カッセイ ニ オヨボス シリョウ ノ エイキ

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Urease activities in the pupal hemolymph were compared in 15 silkworm varieties. In the pupae reared on mulberry leaves, urease activity ranged from 40 to 300mU/ml of hemolymph, while in those reared on an artificial diet no activity was detected in any varieties. Urease activities in the whole body of pupae reared on mulberry leaves ranged from 150 to 200mU per pupa during the pupal period. On the other hand, no activity was detected in the pupae from larvae fed on an artificial diet. In pupae reared on mulberry leaves, urease activities were detected in the alimentary canal, body wall, silk gland and fat body of larvae, alimentary canal of mature larvae at high levels, and further in the malpighian tubes and ovaries in addition to the organs where the activity was recorded in the pupal period. In the eggs laid by adults from larvae reared on mulberry leaves, urease activity was very high, while in those from larvae reared on an artificial diet the activity was hardly detectable. Subsequently, changes of urease activities in the hemolymph during the course of development from the 4th instar larva to adult emergence were compared between both diet groups. In the individuals reared on mulberry leaves, urease remained at a low level during the larval stages, a small increase of activity being observed in the moulting period for the last larval ecdysis. However, at the beginning of spinning, urease activity increased sharply and this high level was maintained in the pupal period and also in the adult. On the other hand, in the hemolymph from the individuals reared on an artificial diet, urease activity was hardly detected throughout the whole stages examined. When the artificial diet coated with jack bean urease was administered orally to the last instar larvae for only 2 days before spinning, urease activity was detected in the pupal hemolymph. Also a high activity of urease was observed in the pupae from silkworms fed on the artificial diet coated with mulberry leaf powder for 2 days. These results suggest that mulberry urease is absorbed through the silkworm intestine. Therefore, the accumulation of urea in the silkworms reared on an artificial diet can be attributed to the lack of urease in the diet.

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