Super-resolution Fluorescence Microscopy in Nano-meter Scale Region Using Two-color Laser Beams
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- IKETAKI Yoshinori
- Olympus Optical Co., Ltd.
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- WATANABE Takeshi
- Institute for Molecular Science
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- SAKAI Makoto
- Institute for Molecular Science
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- ISHIUCHI Shun-ichi
- Institute for Molecular Science
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- OMATSU Takashige
- Department of Information and Imaging Science, Chiba University
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- YAMAMOTO Kimihisa
- Department of Chemistry, Keio University
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- FUJII Masaaki
- Institute for Molecular Science
Bibliographic Information
- Other Title
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- 2色レーザ光を用いたナノ空間超解像蛍光計測法
- 2ショク レーザコウ オ モチイタ ナノ クウカン チョウカイゾウ ケイコウ ケイソクホウ
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Abstract
A super-resolution fluorescence microscopy using two-color laser beams was proposed. The microscopy is based on the combination of two-color fluorescence dip spectroscopy and a phase modulation technique for the laser beam. By applying the proposed technique to a laser-scanning microscope, a fluorescence image of a sample can be observed with a spatial resolution overcoming the optical diffraction limit. To demonstrate validity of the microscopy, we constructed a scanning microscope system using commercial nano-second pulse lasers. An image of micro beads containing dye molecules was observed by the microscopy. We succeeded in obtaining the image with a resolution overcoming the diffraction limit in nano-meter scale region. The experimental data showed that the resolution was improved three times at least. The microscopy is expected to be an appropriate analysis method for the samples with nano-meter scale structures.
Journal
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- Hyomen Kagaku
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Hyomen Kagaku 24 (7), 392-399, 2003
The Surface Science Society of Japan
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Details 詳細情報について
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- CRID
- 1390282681431285504
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- NII Article ID
- 10011228129
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- NII Book ID
- AN00334149
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- COI
- 1:CAS:528:DC%2BD3sXntl2ns7o%3D
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- ISSN
- 18814743
- 03885321
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- NDL BIB ID
- 6645028
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed