Specific Vital Staining of Shizomycetes by Calcofluor White M2R

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  • ISHIDA Nakao
    Department of Bacteriology, Tohoku University School of Medicine
  • KUMAGAI Katsuo
    Department of Bacteriology, Tohoku University School of Medicine
  • KIMURA Mizuo
    Department of Bacteriology, Tohoku University School of Medicine Department of Dermatology, Hirosaki University School of Medicine

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Other Title
  • Calcofluor whiteによる細菌の蛍光染色
  • Calcofluor white ニ ヨル キン ノ ケイコウ センショク 2 キン ノ ゾウショク ニ オヨボス エイキョウ
  • Calcofluor white ニ ヨル サイキン ノ ケイコウ センショク 1 キン ノ ソクジ センショクホウ
  • 第1報 菌の即時染色法

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A basic dye commercially used as the brightener agent in detergents of the washings, , Calcofluor white, was found by Darken to be useful for the vital staining of several bacteria. Our study was further developed in respect to a specificity of a differential staining between animal and plant cells. Several cells of plant origin such as bacteria, Candida and Chlorophyta can be stained by the dye, but all of the tissue culture cells of animal origin so far examined can not be stained at all. When 100mcg/ml of the dye was added to the cells without receiving any fixation procedure, the plant cells can be differentiated from the animal cells, instantaeneously.<br>Although the living bacteria without any fixation are never stained with dyes such as methylene blue, the bacteria labeled with Calcofluor white, which were able to grow and divide, were stained with a solution of methylen blue.

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