Calcofluor whiteによる菌の螢光染色

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タイトル別名
  • Specific Vital Staining of Shizomycetes by Calcofluor White M2R
  • Calcofluor white ニ ヨル キン ノ ケイコウ センショク 2 キン ノ ゾウショク ニ オヨボス エイキョウ
  • II. Growth of the Fluorescing Bacteria
  • (第2報) 菌の増殖に及ぼす影響

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Calcofluor white M2R (CFW), a brightner agent in detergents of washing, was bound by cells of plant origin such as bacteria, Candida and Chlorophyta, but not by the tissue culture cells of animal origin so far examined. The present report is concerned with experiments designed to investigate the transmissibility of the dye to bacterial progenies.<br>When the viable cells of Escherichia coli or Candida albicans in Trypticase soy broth was exposed to CFW at 2, 000 to 2mcg/ml concentrations and incubated, they were instantaeneously stained with the dye, but the dye did not cause any significant growth inhibition of the organisms throughout the incubation period. The fluorescent E. coli was washed with sterile physiological saline and was then transfered to new medium, and followed by the incubation at 37°C. All of the progenies so far examined up to 8 hours showed intense fluorescence. This result suggests that CFW bound to the cells was transferred to their progenies to a detectable extent, at least after 16 repeated divisions.

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