On the Subviral Agents of Rabbit Myxoma and Shope Fibroma Viruses

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  • TAKEHARA Manabu
    Department of Microbiology, School of Medicine, Kobe University Growth Research Institute, Kobe Medical College

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  • ウサギ粘液腫および線維腫ウイルスのSubviral Agentについて
  • ウサギ ネンエキ シュ オヨビ センイ シュ ウイルス ノ Subviral Agent ニ ツイテ

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Abstract

An infective subviral agent (SVA) was isolated from the rabbit kidney (RK) cells infected with rabbit myxoma (South American Sanarelli strain) or Shope fibroma (OA strain) virus, When some properties of the SVA were examined briefly, they differed apparently from typical mature particles of myxoma-fibroma viruses in a few point.<br>Some experimental methods and results obtained are summarized as follows:<br>The SVA of myxoma virus was found in the virus-infected RK cell lysates after infection for 1, 4 and 6 hours. On the other hand, the SVA of fibroma virus was present in the virus-infected cell lysates after infection for 1 and 8 hours. Both myxoma and fibroma SVAs can be completely separated from the typical mature virus particles (about 200-300mμ in diameter) by ultrafiltration through 50mμ Millipore filters.<br>Both myxoma and fibroma SVAs revealed infectivity by being inoculated into the RK cell culture and injected intradermally into rabbits as well as the complete virus particles. Their infectivities were not affected by the action of DNA-ase (2μg/ml) at 37°C for 30 minutes.<br>Infectivity of the SVA from myxoma virus-infected cells was inactivated markedly more than that of myxoma virus by the effect of trypsin (100μg/ml) at 37°C for 30 minutes, and also neutralized completely after incubation at 37°C for 1 hour with anti-myxoma immune rabbit serum. When thermal inactivation rate of both myxoma SVA and virus was determined by incubation at 37°C for 30 minutes, surviving fraction of the SVA was less than 50% as compared with that of myxoma virus.<br>As speculation on these experimental results, the myxoma-fibroma subviral agents appear to contain protein and DNA, but they may be smaller particles than typical myture myxoma-fibroma virus particles. The fact that the myxoma SVA could be detected after infection for 1, 4 and 6 hours seems to be closedly related to time of the growth cycle of this virus. Thus, the SVA which is detectable in earlier stage of the infection may differ from the agent found in later stage of the infection.

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