ウエルシュ菌の分離同定用培地の検討

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  • On the Medium for Isolation and Identification of <i>Clostridium perfringens (welchii)</i>
  • ウエルシュキン ノ ブンリ ドウテイヨウバイチ ノ ケントウ

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In Japan, glucose blood agar plate has been widely used for the isolation of clostridia including Clostridium perfringens. Recently, egg yolk media with Neomycin sulphate were recommended for this purpose. However, the utility of such media is largely dependent on the basal medium as well as the accessory reagents added. We prepared a reproducible medium containing Kanamycin and studied the effects of various constituents of the medium on the isolation of Cl. perfringens. The basal medium consists of 0.5% heart infusion (paste), 1.0% peptone prepared from meat, 1.0% peptone prepared from milk casein, 0.5% NaCl, 1.0% lactose, 0.005% phenol red, 0.02% Kanamycin sulphate and 2.0% agar. The pH was adjusted to 7.6. This basal medium is added with 50% suspension of egg yolk to 10 per cent. Kanamycin should be omitted for the examinations of heated samples, because of the poor growth of the heat treated organisms on the media with Kanamycin.<br>The basal medium has the following advantages:<br>(a) It can be used for egg-yolk agar as well as blood agar, since the dye containing in it does not interfere with the developement of green colour of the colony of Cl. perfringens on the latter medium. (b) No difference was found in the ability to detect the organisms between blood agar and egg yolk agar, so long as this basal medium was used. The results were also comparable with those by cooked meat media. (c) This egg yolk medium may also be used as a medium for identification of Cl. perfringens when combined with the specific antitoxin.

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