ぶどう球菌の耐塩機構の解析　　プロリン含量と含水量の変化による浸透圧調節

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  • Salt-Tolerance Mechanism of <i>Staphylococcus aureus</i>
  • ブドウキュウキン ノ タイエン キコウ ノ カイセキ プロリン ガンリョウ ト
  • プロリン含量と含水量の変化による浸透圧調節

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To elucidate the salt tolerance mechanism of Staphylococcus aureus, the adaptive changes of the contents of amino acids and water in the cell were studied by altering the salt concen-tration of the growth media.<br>Only a trace amount of proline was detected in the cells of S. aureus grown in a medium containing 0.1M NaCl. The cells grown in a medium containing 1.0 and 1.8M NaCl had 428 and 1, 440μmoles proline accumulated, respectively, per g of dry weight of cells. The intracellular water content was 1.70g/g dry wt. when cells were grown in a medium containing 0.1M NaCl. When the cells were transferred to a medium containing 1.8M NaCl, their water content decreased to 0.80g/g dry wt. within one minute, while their intracellular free proline content increased gradually from O to 1, 400μmoles/g dry wt. after incubation at 37C for 30 minutes. Meanwhile their water content increased to 0.88g/g dry wt. The accumulation of proline was found to be due to a temperature-dependent selective uptake from the medium. The accumulated proline was released quickly when the cells were transferred from a high salt medium to a low salt solution. The efflux of proline occurred at 0-4C, suggesting that the process might not be energy-dependent.<br>The role of proline and water content in the osmoregulation mechanism of S. aureus was made clear. The salt-tolerance mechanism was discussed, taking its correlation with adaptive changes in the cell-membrane constituents into consideration.

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