Properties of a Temperate phage of <i>Lactobacillus salivarius</i>

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  • <i>Lactobacillus salivarius</i>のテンペレートファージPLS-1の誘発とその性質
  • Lactobacillus salivarius ノ テンペレートファージ PLS-1 ノ ユウハツ ト ソノ セイシツ

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A phage, PLS-1, derived from Lactobacillus salivarius examined for several properties. Treatment with mitomycin C at a concentration higher than 2mcg/ml or ultraviolet (UV) irradiation using a 15-watt ultraviolet lamp at a distance of 90cm for 30 to 60 seconds (5.4ergs/cm2/sec) was proved to be required for the efficient induction of phage PLS-1 from its original lysogenic strain Sa-S. The “cured” strains derived from strain Sa-S were shown to be effective as indicators for phage PLS-1. Phage PLS-1 had a latent period of approximately 80 minutes and its burst size ranged from 100 to 130. Comparison was made on sensitivity to UV irradiation and serological specificity between phage PLS-1 and some other Lactobacillus phage.

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