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- 前田 照夫
- Animal Reproduction Laboratory, Faculty of Applied Biological Science, Hiroshima University
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- 建本 秀樹
- Animal Reproduction Laboratory, Faculty of Applied Biological Science, Hiroshima University
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- 寺田 隆登
- Animal Reproduction Laboratory, Faculty of Applied Biological Science, Hiroshima University
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- 堤 義雄
- Animal Reproduction Laboratory, Faculty of Applied Biological Science, Hiroshima University
書誌事項
- タイトル別名
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- A convenient method for evaluation of frozen-thawed damage to acrosomes of fowl spermatozoa using a gelatin substrate slide.
- A convenient method for evaluation of frozen-thawed damage to acrosomes of fowl spermatozoa using gelatin substrate slide
説明
The present study was designed to develop a simple and quantitative technique evaluating the extent of freeze damage in a fowl spermatozoon. Acrosin proteolytic activity (APA) by both of non-frozen and frozen-thawed spermatozoa in glucose solution containing various levels of glycerol was measured using a gelatin substrate slide. The mean diameter of 100 halos made by spermatozoal digestion was used as an indication of APA. There was no significant difference in the APA of non-frozen spermatozoa among any groups. After freeze-thawing, the APAs of spermatozoa in all groups decreased remarkably as compared with its of non-frozen spermatozoa. The APA of frozen spermatozoa significantly increased with the increase of glycerol concentration from 0 to 7%, but they decreased significantly according to the increase of glycerol concentration beyond 10%. Therefore, this technique appears to be a more effective and more simplified method for evaluation of freeze-thaw damage of the acrosome in fowl spermatozoa.
収録刊行物
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- 日本家禽学会誌
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日本家禽学会誌 27 (1), 66-71, 1990
日本家禽学会
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詳細情報 詳細情報について
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- CRID
- 1390282681433981184
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- NII論文ID
- 130003622509
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- ISSN
- 00290254
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可