Chicken DNA Fingerprints Using Six Different Probes.

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  • KIMURA Tomoki
    Laboratory of Animal Breeding, Faculty of Agriculture, Kobe University
  • MANNEN Hideyuki
    Graduate School of Science and Technology, Kobe University
  • TSUJI Soichi
    Laboratory of Animal Breeding, Faculty of Agriculture, Kobe University
  • MUKAI Fumio
    Laboratory of Animal Breeding, Faculty of Agriculture, Kobe University
  • GOTO Nobuo
    Laboratory of Animal Breeding, Faculty of Agriculture, Kobe University
  • KANO Noboru
    Hyogo Station, National Livestock Breeding Center, Ministry of Agriculture, Forestry and Fisheries
  • YAMAMOTO Tugito
    Hyogo Station, National Livestock Breeding Center, Ministry of Agriculture, Forestry and Fisheries
  • SATO Tadaaki
    Hyogo Station, National Livestock Breeding Center, Ministry of Agriculture, Forestry and Fisheries

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Other Title
  • 異なるプローブによるニワトリＤＮＡフィンガープリント
  • コトナル プローブ ニ ヨル ニワトリ DNA フィンガー プリント

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Description

Using two lines of chicken, 52 line originated from White Cornish and WL-B line originated from White Leghorn, we examined DNA fingerprints probed with five chemically synthesized repetitive sequences (M13, YNZ22, mo-1, 33.15 and alpha-globin) comparing with that probed with M13 phage repetitive sequence. To make each probe, an oligonucleotide of each repetitive unit less than 20 bases was syn-thesized using a DNA synthesizer with a complementary oligonucleotide, and after phosphorylation they were annealed and ligated. The ligated DNA fragment was amplyfied by PCR using the oligonucleotides as primers. The amplyfied DNA frag-ments are from 0.5 to 20kbp. DNA fingerprints probed with three repetitive sequences of YNZ22, mo-1 and alphaglobin showed clear and well separated numerous bands as well as that probed with M13 phage repetitive sequence. Since the number of bands clearly detectable are increased up to 393 as a total, it is possible to make a linkage analysis between these markers and some qualitative or quantitative traits. DNA fingerprints probed with the repetitive sequence revealed a characteristic pattern in each chicken and in line, indicating that the method described here was useful for parentage analysis and for elucidaing of the genetic structure of a chicken strain.

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