Temperature-Dependent Immobilization and Restoration of Fowl Sperm Motility Caused by Intracellular Free Calcium.

DOI JASI Web Site Web Site 32 References
  • ASHIZAWA Koji
    Laboratory of Animal Reproduction, Faculty of Agriculture, Miyazaki University
  • FUJIYAMA Arata
    Laboratory of Animal Reproduction, Faculty of Agriculture, Miyazaki University
  • TSUZUKI Yasuhiro
    Laboratory of Animal Reproduction, Faculty of Agriculture, Miyazaki University

Bibliographic Information

Other Title
  • 細胞内カルシウム•イオンによる鶏精子の運動調節
  • Temperature-Dependent Immobilization and Restoration of Fowl Sperm Motility Caused by lntracellular Free Calcium

Search this article

Abstract

Fowl spermatozoa maintained vigorous movement at 30°C with or without Ca2+. In contrast, the motility was reversibly inhibited at 40°C without Ca2+, but could be immediately restored by the addition of Ca2+. However, the addition of verapamil, a specific Ca2+ channel blocker, before the addition of Ca2+, could not obtain fully motile spermatozoa at 40°C . Under these conditions, the intracellular free Ca2+ concentration was lower than that of the control (no addition of verapamil), as measured by a fluorescent Ca2+ indicator, fura-2. The preparations of spermatozoa without Ca2+ were in-cubated at 30°C, and then transferred and incubated at 40°C . After that, they were re-incubated at 30°C again. During the second incubation at 30°C, high motility was obtained, even in the presence of verapamil. The addition of Sr2+, which appears to stimulate the release of Ca2+ from the mitochondria, was also effective for the stimulation of motility at 40°C, and induced a concomitant increase in the intracellular free Ca2+ concentrations. These results suggest that intracellular free Ca2+ play a pivotal role in regulating fowl sperm motility. Furthermore, it appears that fowl sperm mitochondria have a significant role in controlling intracellular free Ca2+ concentrations. This mech-anism, rather than Ca2+ fluxes through the plasma membrane, may be involved in the temperature-dependent immobilization and restoration of fowl sperm motility.

Journal

References(32)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top