Sex identification in chickens by means of the presence of the W chromosome-specific repetitive DNA units.

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  • KAGAMI Hiroshi
    Laboratory of Animal Genetics, Faculty of Agriculture, Nagoya University
  • NAKAMURA Hiromu
    Molecular Biology Unit, Aichi Cancer Center, Research Institute
  • TOMITA Takeshi
    Laboratory of Animal Genetics, Faculty of Agriculture, Nagoya University

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  • W染色体に特異的な反復DNA配列の検出によるニワトリの性判別

Abstract

The W chromosome specific repetitive DNA units were detected easily by the XhoI digestion of genomic DNA, purified from a female whole blood sample, but could not be detected in male. Since the red blood cells of the chicken are nucleated, it is possible to obtain enough amounts of DNA preparation from a small volume of whole blood without sacrificing the animal. The detection of the female-specific repetitive DNA units is simple, reliable and efficient. By applying this technique, the identification of the sex in chickens was easily achived.

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