鶏における非ステロイド性アロマターゼインヒビターの性転換効果と性腺アロマターゼmRNA発現

DOI 日本農学文献記事索引 Web Site Web Site 被引用文献7件 参考文献17件
  • アビナワント
    名古屋大学農学部資源生物環境学科動物機能制御学講座
  • 島田 清司
    名古屋大学農学部資源生物環境学科動物機能制御学講座
  • 齋藤 昇
    名古屋大学農学部資源生物環境学科動物機能制御学講座

書誌事項

タイトル別名
  • Sex-Reversal Effects of Non-Steroidal Aromatase Inhibitor on Aromatase (P450arom) mRNA Expression in Adult Chicken Gonads.
  • Sex-Reversal Effects of Non-Steroidal A

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This study was conducted to investigate the effects of non-steroidal aromatase inhibitor (AI, Fadrozole, Ciba-Geigy) on gonadal differentiation in the chicken. Fertilized eggs of single comb White Leghorn hens were injected with AI on day 5 of incubation. AI-treated eggs were incubated, hatched, and raised until 10 months of age. The genetic sex of the adult chickens was evaluated by Southern hybridization analysis of red blood cell lysate using 32P-dCTP-labelled W-specific DNA probe. Groups were (1) hens without a sex-reversal effect, (2) sex-reversed hens, genetic females but phenotypic males, and (3) normal genetic males displaying no obvious changes in secondary sex characteristics. Hens without a sex reversal effect had normal ovarian follicles and a well-developed oviduct on the left side. Genetic males without sex-reversal effect had a pair of normal testes containing seminiferous tubules and interstitial cells. Numerous sperm were present in the seminiferous tubules. Sex-reversed females had retarded gonads on both sides. These gonads contained essentially the same cellular components as those of normal testes, however, the inside diameter of the seminiferous tubules was extremely small. Sex-reversed females had the secondary sex characteristics of the male and displayed mating behavior of normal cocks but had a developed oviduct as well. Plasma concentrations of testosterone and estradiol of the sex-reversed females showed intermediate levels between those of genetic and phenotypic females and males. The gonadal mRNA levels of P45017α-hydroxylase were higher in the genetic males than in phenotypic females and sex-reversed females. On the other hand, mRNA levels of P 450aromatase were higher in the phenotypic females than the sex-reversed females and genetic males. These studies indicate that AI may cause reduction of mRNA expression of P450aromatase and plasma levels of estradiol of sex-reversed females which are indirectly correlated with retarded development of the gonads.

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