大量死を伴う異常アコヤガイの血球像の変化

  • 前野 幸男
    独立行政法人水産総合研究センター養殖研究所南勢庁舎 独立行政法人国際農林水産業研究センター水産部
  • 伊東 尚史
    独立行政法人水産総合研究センター養殖研究所玉城分室
  • 釜石 隆
    独立行政法人水産総合研究センター養殖研究所南勢庁舎
  • 森実 庸男
    愛媛県水産試験場
  • 中島 員洋
    独立行政法人水産総合研究センター養殖研究所玉城分室

書誌事項

タイトル別名
  • Morphological Changes of Hemocytes in Hemolymph Smear Preparations of Diseased Japanese Pearl Oyster Pinctada fucata martensii with Mass Mortality.
  • タイリョウシ オ トモナウ イジョウ アコヤガイ ノ ケッキュウゾウ ノ ヘンカ

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抄録

In diseased Japanese pearl oyster Pinctada fucata martensii collected from Ehime, Japan from April to July 1998 where mass mortalities of cultured pearl oyster had occurred since 1996, histopathological features such as atrophy of the connective tissue in the mantle and adductor muscle were commonly observed. These histopathological changes were also reproduced in experimental pearl oyster by inoculation with hemolymph of diseased pearl oyster. Morphological changes of hemocytes in naturally and experimentally infected Japanese pearl oyster were examined. In naturally infected oyster, enlarged cells and the presence of granules and vacuoles in the cytoplasm of hemocytes were observed in hemolymph smear preparations stained with May Grunwald-Giemsa, and these changes were consistent with the histopathological changes. In experimentally infected oyster, these morphological changes of hemocytes were also correlated with the progression of histopathological changes. These results suggest that the observation of morphological changes of hemocytes in hemolymph smear preparations can be a rapid and convenient method for presumptive diagnosis of the disease occurring in Japanese pearl oyster.

収録刊行物

  • 魚病研究

    魚病研究 36 (4), 225-230, 2001

    日本魚病学会

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