A Sensitive Enzymatic Determination of Acylglycerol Hydroperoxides by Lipase and Glutathione Peroxidase with N-(9-Acrydinyl)maleimide fluorometry.
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- KAMATA Tsuneo
- Division of Food and Nutrition, Jyumonji Gakuen Women's College
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- TANNO Hiroyuki
- Central Research Laboratory, Ajinomoto Co., Inc.
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- OHRUI Hiroshi
- Department of Food Chemistry, Faculty of Agriculture, Tohoku University
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- MEGURO Hiroshi
- Department of Food Chemistry, Faculty of Agriculture, Tohoku University
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説明
N-(9-Acrydinyl)maleimide (NAM) fluorometry was applied to the enzymatic determination of mono-, di- and triacylglycerol hydroperoxides (HPOs) by glutathione peroxidase (GPX) and lipase. Lipase hydrolysis made it possible to determine the acylglycerol HPOs. Two enzyme reactions were conducted simultaneously in one pot for 4hr at 25°C in 0.1 M phosphate buffer of pH 7.5 in a nitrogen atmosphere in the dark. The released glutathione-SH was measured by NAM fluorometry, the calibration curve giving a linear relationship in the range from 0.1 to 10nmol of HPO with a sample size of 0.2 to 20 mg. The sensitivity was about 250 times higher than that by conventional iodometry.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 55 (8), 1989-1992, 1991
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681436595968
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- NII論文ID
- 110006325671
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- NII書誌ID
- AA00515312
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- ISSN
- 18811280
- 00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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