Vibrio anguillarumの菌体外産物の毒性

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タイトル別名
  • Toxicity of extracellular products of Vibrio anguillarum.
  • Vibrio anguillarumの菌体外産物の毒性〔英文〕
  • Vibrio anguillarum ノ キンタイガイ サンブツ ノ ドクセイ

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Vibrio anguillarum produced heat labile(70°C, 10 min) exotoxin lethal to goldfish, Japanese eel, ayu and mouse when injected. The extracellular products(ECP)from cellophane plate cultures(nutrient agar or BHI agar)at 20°Cor 25°Cfor 18-36 h were toxic, but not in 24 h BHI broth culture at 25°C. No lethal endotoxin was detected in filtrate of sonicated cells or heated cells obtained from cellophane plate culture.The lethal dose of the crude ECP from a high virulent strain was approximately 15μg protein/g fish(goldfish).<BR>Proteolytic activity of ECP, which was measured by using azocasein as reaction substrate, was re-cognized to coexist together with lethal toxicity. When virulent strains were compared with low virulent strains, the production of the lethal exotoxin and protease well correlated with the virulence.

収録刊行物

  • 魚病研究

    魚病研究 19 (2), 89-96, 1984

    日本魚病学会

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