サケ科魚類における細菌性冷水病菌の卵内感染機序
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- Kumagai Akira
- Miyagi Prefecture Fisheries Technology Institute
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- Nawata Akatsuki
- Miyagi Prefecture Fisheries Technology Institute Freshwater Fisheries Experimental Station
書誌事項
- タイトル別名
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- Mode of the Intra-ovum Infection of Flavobacterium psychrophilum in Salmonid Eggs
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The mode of intra-ovum infection by Flavobacterium psychrophilum, which causes bacterial cold-water disease and rainbow trout fry syndrome, was investigated in eggs of rainbow trout Oncorhynchus mykiss. When newly spawned unfertilized eggs were immersion-challenged with F. psychrophilum at concentrations of 107 CFU/mL or higher and subsequently inseminated and water-hardened, the bacteria were detected in the egg contents. The infection rate in eggs was related to the bacterial concentration in a dose-dependent manner. When mature female rainbow trout were intraperitoneally injected with F. psychrophilum (3.3 × 109 CFU/fish) 5-9 days prior to ovulation, intra-ovum infection was found in eggs spawned from the fish showing heavy contamination of the bacterium in the ovarian fluid (> 106 CFU/mL). These results indicate that F. psychrophilum can contaminate the surface of eggs in ovarian fluid and enter eggs during water-hardening. Live bacterial counts within experimentally infected eggs and immuno-staining observations on the eggs suggested that F. psychrophilum passively entered into the eggs (< 10 CFU/egg) and subsequently grew to over 107 CFU/egg in the perivitelline space by the eyed egg stage. Multiplication of the bacterium in the perivitelline space did not affect the survival of eggs.
収録刊行物
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- 魚病研究
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魚病研究 45 (1), 31-36, 2010
日本魚病学会
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詳細情報 詳細情報について
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- CRID
- 1390282681436963584
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- NII論文ID
- 130000257278
- 10026106101
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- NII書誌ID
- AN00063165
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- ISSN
- 18817335
- 0388788X
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- NDL書誌ID
- 10634010
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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