Enzyme Electrophoresis, Catalase Test and PCR-RFLP Analysis for the Typing of Edwardsiella tarda.

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  • Yamada Yoshiyukim
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Wakabayashi Hisatsugu
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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  • 酵素電気泳動法, カタラーゼテストおよび PCR-RFLP 解析による Edwardsiella tarda の型別
  • Enzyme electrophoresis catalase test an

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Abstract

Enzyme electrophoresis for superoxide dismutase (SOD) and catalase divided 144 Edwardsiella tarda strains into two groups; group 1 contained all the strains isolated from diseased fish in Japan and group 2 included 25 strains from non-disease sources and reference strain ATCC15947T. These groups could be similarly distinguished by a simple catalase test; group 1 showed an instantaneous bubbling, whereas in group 2 bubbling was delayed or weak. PCR-RFLP analysis usingHhaI revealed that the two groups differed in their 16S rDNA genotypes. Furthermore, this analysis subdivided group 1 into two genotypes.

Journal

  • Fish Pathology

    Fish Pathology 33 (1), 1-5, 1998

    The Japanese Society of Fish Pathology

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