Rapid and Simple Method for Detection of Yersinia ruckeri, the Causal Agent of Enteric Redmouth Disease

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  • Sakai Takamitsu
    Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency
  • Oseko Norihisa
    Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency
  • Iida Takaji
    Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency

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Other Title
  • レッドマウス病原因菌の簡易迅速検出法について
  • レッドマウスビョウ ゲンインキン ノ カンイ ジンソク ケンシュツホウ ニ ツイテ

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Description

Availability of known selective-differential culture media and PCR methods for the detection of Yersinia ruckeri, the causal agent of enteric redmouth disease, was investigated. The attempted three selective-differential culture media had some difficulties in the selective growth of Y. ruckeri and the differentiation of the bacterium from other fish-pathogenic bacteria. Three PCR methods targeting 16S rDNA were evaluated to be effective in sensitivity and specificity for the detection of Y. ruckeri. The rapid and accurate diagnosis of redmouth disease will be performed by PCR following Gram stain and cytochrome-oxidase test.

Journal

  • Fish Pathology

    Fish Pathology 41 (3), 127-130, 2006

    The Japanese Society of Fish Pathology

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