Pathogenicity of the Epizootic Ulcerative Syndrome (EUS)-Associated Rhabdovirus to Snakehead Ophicephalus striatus.

DOI Web Site 27 References

Bibliographic Information

Other Title
  • 流行性潰瘍症候群(EUS)の魚から分離されたラブドウイルスのスネークヘッドに対する病原性

Search this article

Abstract

The rhabdoviruses isolated from EUS-affected snakeheads in the Philippines was tested for pathogenicity to healthy, naive snakehead Ophicephalus striatus fry, fingerlings and juveniles. Virus exposure of naive snakehead fry and fingerlings by bath at 20-22.5°C resulted in significant mortalities (p<0.01) with no apparent lesions. Naive snakehead juveniles when injected intramuscularly (IM) with the EUS-associated rhabdovirus at ambient water temperature (28-32°C) did not develop any lesion. However, similarly treated snakehead juveniles at 20-22.5°C developed dermal lesions 3-5 days following treatment. The lesions progressed from slight to moderately advanced lesions by days 10-12 but not to deep ulcers as exhibited by naturally EUS-affected snakeheads. Mean mortalities were higher in the virus-injected fish (72%) compared to those in controls (33%). Moreover, the virus was reisolated from fish in the 20-22.5°C treatment but not from fish in the 28-32°C treatment. Virus from infected tissue filtrate and the virus passaged 3 or 4 times induced similar dermal lesions if the rhabdovirus concentration was 103 TCID50/fish or higher. When administered orally, by bath, by intraperitoneal (IP), IM and subdermal injections to snakehead juveniles, only the latter two viral routes induced dermal lesions. However, IP injection of the rhabdovirus caused 75% mortalities but none in control fish. The results demonstrate the pathogenicity of the rhabdovirus isolates to naive snakeheads at low (20-22.5°C) rearing water temperatures.

Journal

  • Fish Pathology

    Fish Pathology 36 (2), 57-66, 2001

    The Japanese Society of Fish Pathology

References(27)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top