養殖ウナギの主要細菌感染症の迅速診断システムに関する研究―I

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書誌事項

タイトル別名
  • Studies on Rapid Diagnosis System of Main Bacterial Diseases of Pond-cultured Eels in Japan-I
  • ヨウショク ウナギ ノ シュヨウ サイキン カンセンショウ ノ ジンソク シン
  • Basic Investigations on the Diagnosis of Edwardsiellosis by Direct Immunofluorescence
  • 免疫螢光直接法によるパラコロ病診断の基礎的検討

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抄録

Epizootics of edwardsiellosis has been frequently occurring among pond-cultured eels, Anguilla japonica in Japan. Accurate diagnosis of the disease by means of conventional cultural method requires a day or more for completion. In this report, basic investigations for the application of direct immunofluorescence as rapid diagnostic tools of the disease were made.<BR>Antiserum was obtained from rabbit immunized with formalin-killed E. tarda (strain E-8) isolated from eel. The titer against formalin-killed homologous antigen was 1: 2048 by agglutination test. IgG of the antiserum was fractionated by ammonium sulfate precipitation and conjugated with FITC. The F/P molar ratio of labeled antibody was determined as 1.3.<BR>The labeled antibody had staining titer of 1:16 or 1:32 against 18 strains of E. tarda, including homologous strain E-8, isolated from pond-cultured eels at various regions in Japan. But no crossreaction was observed when 2 strains of V. anguillarum, 3 of Vibrio sp., 3 of A. liquefaciens, 8 of Aeromonas sp., one of Ps. anguilliseptica, one of Ps. fluorescens, one of Ps. putida, one of Escherichia coli, 2 of Salmonella sp., one of S. marcescens and one of F. columnaris were stained.<BR>The specific fluorescence of target antigen (E. tarda) was observed when acetone-fixed impression preparations of liver, kidney, spleen and injection site of muscle derived from artificially infected eel were stained with 4-fold dilution of labeled antibody. The specificity of the reaction on impression preparation was ascertained by one-step inhibition test. Stainability of E. tarda on impression preparation was stable for at least 11 days at 37°C. Stainability of E. tarda on impression preparation of liver tissue that had been preserved in 10% formalin for at least 7 days, though became slightly weaker, was not disappeared.

収録刊行物

  • 魚病研究

    魚病研究 15 (1), 49-55, 1980

    日本魚病学会

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