書誌事項
- タイトル別名
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- Studies on Rapid Diagnosis System of Main Bacterial Diseases of Pond-cultured Eels in Japan-I
- ヨウショク ウナギ ノ シュヨウ サイキン カンセンショウ ノ ジンソク シン
- Basic Investigations on the Diagnosis of Edwardsiellosis by Direct Immunofluorescence
- 免疫螢光直接法によるパラコロ病診断の基礎的検討
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説明
Epizootics of edwardsiellosis has been frequently occurring among pond-cultured eels, Anguilla japonica in Japan. Accurate diagnosis of the disease by means of conventional cultural method requires a day or more for completion. In this report, basic investigations for the application of direct immunofluorescence as rapid diagnostic tools of the disease were made.<BR>Antiserum was obtained from rabbit immunized with formalin-killed E. tarda (strain E-8) isolated from eel. The titer against formalin-killed homologous antigen was 1: 2048 by agglutination test. IgG of the antiserum was fractionated by ammonium sulfate precipitation and conjugated with FITC. The F/P molar ratio of labeled antibody was determined as 1.3.<BR>The labeled antibody had staining titer of 1:16 or 1:32 against 18 strains of E. tarda, including homologous strain E-8, isolated from pond-cultured eels at various regions in Japan. But no crossreaction was observed when 2 strains of V. anguillarum, 3 of Vibrio sp., 3 of A. liquefaciens, 8 of Aeromonas sp., one of Ps. anguilliseptica, one of Ps. fluorescens, one of Ps. putida, one of Escherichia coli, 2 of Salmonella sp., one of S. marcescens and one of F. columnaris were stained.<BR>The specific fluorescence of target antigen (E. tarda) was observed when acetone-fixed impression preparations of liver, kidney, spleen and injection site of muscle derived from artificially infected eel were stained with 4-fold dilution of labeled antibody. The specificity of the reaction on impression preparation was ascertained by one-step inhibition test. Stainability of E. tarda on impression preparation was stable for at least 11 days at 37°C. Stainability of E. tarda on impression preparation of liver tissue that had been preserved in 10% formalin for at least 7 days, though became slightly weaker, was not disappeared.
収録刊行物
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- 魚病研究
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魚病研究 15 (1), 49-55, 1980
日本魚病学会
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詳細情報 詳細情報について
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- CRID
- 1390282681437380864
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- NII論文ID
- 130000860544
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- NII書誌ID
- AN00063165
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- ISSN
- 18817335
- 0388788X
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- NDL書誌ID
- 2187828
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- データソース種別
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- JaLC
- NDL
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- 使用不可