種々の方法による組織切片中のRenibacterium salmoninarumの検出－特に間接免疫ペルオキシダーゼ組織染色法を考慮した比較検討

DOI Web Site
  • HOFFMANN R. W.
    Institut für Zoologie und Hydrobiologie, University of Munich, FRG
  • BELL G. R.
    Department of Fisheries and Oceans, Biological Sciences Branch, Pacific Biological Station
  • PFEIL-PUTZIEN C.
    Institut für Zoologie und Hydrobiologie, University of Munich, FRG
  • OGAWA M.
    Institut für Zoologie und Hydrobiologie, University of Munich, FRG

書誌事項

タイトル別名
  • Detection of Renibacterium salmoninarum in tissue sections by different methods - A comparative study with special regard to the indirect immunohistochemical peroxidase technique.
  • 種々の方法による組織切片中のRenibacterium salmoninarumの検出--特に間接免疫ペルオキシダーゼ組織染色法を考慮した比較検討〔英文〕
  • シュシュ ノ ホウホウ ニ ヨル ソシキ セッペンチュウ ノ Renibact

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抄録

Various staining methods (the Gram and periodic acid stains;the indirect fluorescence antibody technique and the indirect peroxidase procedure) were compared for their ability to detect the kidney disease bacterium (Renibacterium salmoninarum) in the tissues of rainbow trout (Salmo gairdneri) fixed by various methods (fresh frozen tissue, frozen formalin-fixed tissue, formalin-or Bouin's-fixed paraffin-embedded tissue). The results demonstrated that only the indirect peroxidase technique gave positive results regardless of the fixation method used to prepare the tissue. The other methods were either less sensitive or yielded negative results after some tissue fixation methods.

収録刊行物

  • 魚病研究

    魚病研究 24 (2), 101-104, 1989

    日本魚病学会

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