Phage Therapy against .BETA.-hemolytic Streptococcicosis of Japanese Flounder Paralichthys olivaceus
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- Matsuoka Satoru
- Ehime Prefectural Chuyo Fisheries Experimental Station
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- Hashizume Takaya
- Graduate School of Biosphere Science, Hiroshima University
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- Kanzaki Hiroyuki
- Saga Prefectural Genkai Fisheries Research and Development Center
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- Iwamoto Emi
- Graduate School of Biosphere Science, Hiroshima University
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- Se Chang Park
- College of Veterinary Medicine, Seoul National University
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- Yoshida Terutoyo
- Faculty of Agriculture, University of Miyazaki
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- Nakai Toshihiro
- Graduate School of Biosphere Science, Hiroshima University
Bibliographic Information
- Other Title
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- ヒラメのβ溶血性レンサ球菌症に対するファージ治療試験
- ヒラメ ノ ベータ ヨウケツセイ レンサ キュウキンショウ ニ タイスル ファージ チリョウ シケン
- Phage Therapy against β-hemolytic Streptococcicosis of Japanese Flounder Paralichthys olivaceus
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Abstract
We examined the therapeutic effect of Streptococcus iniae phages isolated from fish culture environments against experimental streptococcicosis of Japanese flounder Paralichthys olivaceus. Phage sensitivity tests with a double agar method revealed that 31 of 35 S. iniae strains from the flounder have a similar sensitivity to six phage isolates. In phage therapy experiments, fish were injected intraperitoneally (IP) with S. iniae PSi402 and 1 h later IP-injected with a mixture of two or four phage isolates, and observed at 25°C for 2 wk. Mortalities of fish receiving phages were significantly lower than those of control fish without phage-treatment in all four trials. The effect of phage treatment was also demonstrated even at 24 h post-infection, when cell numbers of S. iniae were 107.4 and 104.5 CFU/g in the kidneys and brains of fish, respectively. However, as phage-resistant S. iniae were frequently isolated from dead fish in the phage-treated group, further investigations are required to establish phage therapy of the disease.
Journal
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- Fish Pathology
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Fish Pathology 42 (4), 181-189, 2007
The Japanese Society of Fish Pathology
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Keywords
Details 詳細情報について
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- CRID
- 1390282681437497728
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- NII Article ID
- 10020025016
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- NII Book ID
- AN00063165
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- ISSN
- 18817335
- 0388788X
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- NDL BIB ID
- 9318255
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed