Immersion vaccination and water-borne challenge of ayu (Plecoglossus altivelis) against vibriosis.

DOI Web Site 1 Citations
  • KAWANO Katsuhiko
    Department of Fisheries, Faculty of Agriculture, Miyazaki University Central Laboratories, Kyoritsu Shoji Co. Ltd.
  • AOKI Takashi
    Department of Fisheries, Faculty of Agriculture, Miyazaki University
  • KITAO Tadatoshi
    Department of Fisheries, Faculty of Agriculture, Miyazaki University

Bibliographic Information

Other Title
  • アユのビブリオ病に対する浸せきワクチンと ワクチン魚の高張液攻撃試験法について
  • アユのビブリオ病に対する浸せきワクチンとワクチン魚の高張液攻撃試験法について〔英文〕
  • アユ ノ ビブリオビョウ ニ タイスル シンセキ ワクチン ト ワクチンギョ

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Description

In a Vibrio anguillarum strain passaged through ayu (Plecoglossus altivelis), the LD50 value against ayu by the water-borne challenge method did not change from that of the original strain freshly isolated from diseased fish. The ayu were reproducibly infected with the passaged strain by the water-borne method in which the fish were first exposed to 5.32% NaCl solution and then to a suspension of V. anguillarum cells. The symptoms in ayu infected by this challenge method were observed to be very similar to the symptoms of a natural vibriosis infection. The LD50 value against fish exposed first to NaCl solution and then to the cell suspension was lower than that of fish exposed to only the cell suspension. This challenge method, incorporating initial exposure to a 5.32% NaCl solution followed by exposure to a Vibrio cell suspension, provided a clear difference in mortality between vaccinated and non-vaccinated fish.<BR>High level protection against vibriosis was observed in fish vaccinated by immersion in V. anguillarum cells which had been formalinized, washed, and pelleted or which had been formalinized, washed, and lyophilized.

Journal

  • Fish Pathology

    Fish Pathology 18 (3), 143-149, 1983

    The Japanese Society of Fish Pathology

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