Detection and Characterization of Precipitating Antibody in the Serum of Immature Yellowtail Immunized with Pasteurella piscicida Cells

DOI Web Site

Bibliographic Information

Other Title
  • Ｐａｓｔｅｕｒｅｌｌａ　ｐｉｓｃｉｃｉｄａで免疫したハマチの血清中における沈降抗体の検出と特性
  • Pasteurella piscicida デ メンエキシタ ハマチ ノ ケッ

Search this article

Description

Precipitating antibody against crude lipopolysaccharide (LPS) of Pasteurella piscicida in the serum of immature yellowtail, Seriola quinqueradiata, immunized with the cellular antigen was demonstrated by gel diffusion method (Fig.1).The serum from immunized yellowtail showed a precipitin line formation when the specific soluble antigen, LPS was reacted against the fish serum after electrophoresis. The single line occurred in the μ-globulin region identified to yellowtail immunoglobulin (Fig. 2).The precipitating antibody activity was detected in the high molecular weight fraction of gel filtration on Sepharose 6B (Fig.3).<BR>It is concluded that precipitating antibody produced in the serum of immature yellowtail corresponds with macroglobulin in young fish.

Journal

  • Fish Pathology

    Fish Pathology 17 (2), 125-127, 1982

    The Japanese Society of Fish Pathology

Keywords

Details 詳細情報について

Report a problem

Back to top