Disinfection of Fertilized Grouper Eggs with Electrolyzed Seawater for Prevention of Viral Nervous Necrosis

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  • ウイルス性神経壊死症の防除を目的とした電解海水によるクエおよびマハタ受精卵の消毒条件の検討
  • ウイルスセイ シンケイ エシショウ ノ ボウジョ オ モクテキ ト シタ デンカイ カイスイ ニ ヨル クエ オヨビ マハタ ジュセイラン ノ ショウドク ジョウケン ノ ケントウ

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Abstract

Conditions of fertilized egg disinfection with electrolyzed seawater were optimized to prevent vertical transmission of betanodavirus in longtooth grouper Epinephelus bruneus and sevenband grouper E. septemfasciatus. The treatments were performed by immersing eggs in electrolyzed seawater containing 0 to 1.5 mg/L of free chlorine. The conditions which showed no adverse effect on hatching rates of both species were 0.3 to 0.5 mg/L of free chlorine for 5 min, 1.0 mg/L for 3 min and 1.5 mg/L for 1 min. More than 99.4% of betanodavirus (SGNNV) was inactivated by the treatments of 0.3 to 1.0 mg/L of free chlorine for 3 min and 1.5 mg/L for 1 min.

Journal

  • Fish Pathology

    Fish Pathology 48 (1), 5-8, 2013

    The Japanese Society of Fish Pathology

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