Application of Tissue Clearing Techniques to 3D Study of Infectious Disease Pathology in Fish

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  • Ohnuma Shimon
    Department of Marine Biosciences, Tokyo University of Marine Science and Technology
  • Katagiri Takayuki
    Department of Marine Biosciences, Tokyo University of Marine Science and Technology
  • Maita Masashi
    Department of Marine Biosciences, Tokyo University of Marine Science and Technology
  • Endo Makoto
    Department of Marine Biosciences, Tokyo University of Marine Science and Technology
  • Futami Kunihiko
    Department of Marine Biosciences, Tokyo University of Marine Science and Technology

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抄録

Conventional fish pathological diagnoses are limited in ability to detect pathogens in infected fish due to limitations of partial or 2 dimensional images. Here, we tested three tissue clearing techniques (ClearT, SeeDB and CUBIC), which make the fish body transparent, allowing visualization of pathogens. CUBIC was most effective in clearing the fish body, and was applicable to immunohistochemistry and GFP (green fluorescent protein) imaging. Furthermore, DAPI-labeled Aeromonas hydrophila was visible under a fluorescence microscope. Thus, CUBIC is the most suitable tissue clearing technique allowing 3 dimensional analysis of infectious diseases in fish.

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  • 魚病研究

    魚病研究 52 (2), 96-99, 2017

    日本魚病学会

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