Rapid and Simple Detection Method of "Candidatus Xenohaliotis californiensis" Using Fecal PCR in Abalone Haliotis discus discus and H. gigantea

DOI Web Site 被引用文献2件 参考文献14件
  • Kiryu Ikunari
    National Research Institute of Aquaculture, Fisheries Research Agency
  • Nishioka Toyohiro
    National Research Institute of Aquaculture, Fisheries Research Agency
  • Yuasa Kei
    National Research Institute of Aquaculture, Fisheries Research Agency
  • Kurita Jun
    National Research Institute of Aquaculture, Fisheries Research Agency
  • Shimahara Yoshik
    National Research Institute of Aquaculture, Fisheries Research Agency
  • Ototake Mitsuru
    National Research Institute of Aquaculture, Fisheries Research Agency
  • Ikegami Naoya
    Chiba Prefectural Fisheries Research Center
  • Oseko Norihisa
    National Research Institute of Aquaculture, Fisheries Research Agency

書誌事項

タイトル別名
  • Rapid and Simple Detection Method of “<i>Candidatus </i>Xenohaliotis californiensis” Using Fecal PCR in Abalone <i>Haliotis discus discus</i> and <i>H. gigantea</i>
  • Rapid and Simple Detection Method of ^|^ldquo;Candidatus Xenohaliotis californiensis^|^rdquo; Using Fecal PCR in Abalone Haliotis discus discus and H. gigantea

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抄録

We developed a simple PCR-based method using feces to detect “Candidatus Xenohaliotis californiensis” (Rickettsia-like organism, RLO), which is responsible for withering syndrome (WS). Four abalone groups (Haliotis discus discus and H. gigantea) naturally or artificially infected with WS-RLO were prepared. After daily collection of the feces from individual animals over a seven-day period, the posterior esophagus (PE) was excised, and subjected to PCR for WS-RLO. WS-RLO-positive results were obtained from the feces of 30-67% animals, and from the PE of 17-97% animals. For the fecal PCR, more than one animal was positive from each group every day, although the WS-RLO-positive rate daily varied. When the sensitivity of the PCR was compared between DNA extracted from feces by boiling and that by QIAamp® DNA Stool Kit, there was no difference between the two methods. Fecal PCR combined with boiling DNA extraction is rapid and simple for screening abalone groups infected with WS-RLO.

収録刊行物

  • 魚病研究

    魚病研究 49 (2), 41-48, 2014

    日本魚病学会

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