シロサケにおける伝染性造血器壊死症ウイルス（IHNV）M遺伝子RNAと相同のDNA型の存在
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- Suzuki Kunio
- Eastern Hokkaido Inland Water Fisheries Section, The Hokkaido Fish Hatchery
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- Sakai D. K.
- Faculty of Bio-industry, Tokyo University of Agriculture
書誌事項
- タイトル別名
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- Presence of Homologous DNAs to M Gene RNA of Infectious Hematopoietic Necrosis Virus in Chum Salmon Oncorhynchus keta
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説明
Homologous DNAs (M DNA) to the infectious hematopoietic necrosis virus (IHNV) M gene were detected in river-homing chum salmon Oncorhynchus keta sperm, eyed-egg embryos, alevins and fry collected from nine rivers and four hatcheries in Hokkaido Prefecture, Japan, in 1998-2002, although no virus titer was observed in the samples. Real-time PCR assay revealed M DNA at the highest number of 1.7 × 104 copies/mg of tissue. Nucleotide sequence similarities between the M DNA and the viral M genes were ≥97%. When CHSE-214 and RTG-2 cells were inoculated with IHNV, the cells contained 3.8 × 101 to 1.8 × 103 copies of M DNA/105 cells and showed cytopathic effects (CPE). Salmonid fish fry and yearlings infected with the virus contained 1.3 × 101 to 2.6 × 103 copies of M DNA/mg of tissue. CHSE-214 cells transfected with the viral RNA produced M DNA without showing CPE. Reverse transcriptase (RTase) activities in the fish cells and the fish tissues were equivalent to 86-578 pg HIV-1 RTase/106 cells and 2.1-12 pg HIV-1 RTase/mg of tissue, respectively. These results suggest that M DNA is produced from IHNV RNA and retained in the fish.
収録刊行物
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- 魚病研究
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魚病研究 44 (1), 24-32, 2009
日本魚病学会
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詳細情報 詳細情報について
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- CRID
- 1390282681438435840
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- NII論文ID
- 130000104504
- 10025151338
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- NII書誌ID
- AN00063165
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- ISSN
- 18817335
- 0388788X
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- NDL書誌ID
- 10226779
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
