Identification and Molecular Characterization of Z/ZE Lineage MHC Class I Heavy Chain Homologue and β2-microglobulin from Rock Bream Oplegnathus fasciatus
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- Nikapitiya Chamilani
- Department of Aqualife Medicine, Chonnam National University
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- Jung Sung-Ju
- Department of Aqualife Medicine, Chonnam National University
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- Jung Myung-Hwa
- Department of Aqualife Medicine, Chonnam National University Aquatic Animal Hospital, Chonnam National University
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- Song Jun-Young
- National Fisheries Research and Development Institute
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- Lee Jehee
- Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University
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- Lee Jeong-Ho
- Genetics and Breeding Research Center, National Fisheries Research and Development Institute
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- Oh Myung-Joo
- Department of Aqualife Medicine, Chonnam National University
書誌事項
- タイトル別名
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- Identification and Molecular Characterization of Z/ZE Lineage MHC Class I Heavy Chain Homologue and β2-microglobulin from Rock Bream <i>Oplegnathus fasciatus</i>
- Identification and Molecular Characterization of Z/ZE Lineage MHC Class I Heavy Chain Homologue and ^|^beta;2-microglobulin from Rock Bream Oplegnathus fasciatus
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We identified and characterized full-length cDNAs of rock bream Oplegnathus fasciatus major histocompatibility complex (RbMHC) class I and β2-microglobulin (Rbβ2m) molecules. The full-length RbMHC class I cDNA was 2,667 bp with a 1,227 bp open reading frame encoding 409 amino acids (aa). The Rbβ2m full-length cDNA (1,339 bp) consisted of a 354 bp coding region, encoding 118 aa. RbMHC class Iα was clustered with the Z/ZE lineage of known MHC class I sequences, suggesting a novel molecule belonging to the Z/ZE lineage. Although characteristic features (residues and motifs) known to be conserved in classical and non-classical class I molecules were conserved in RbMHC class Iα with few modifications, it is more closely resembled to classical MHC class I. RbMHC class I and Rbβ2m were transcribed constitutively in healthy fish, but expression differed among the different organs and was highest in whole blood. Low sequence variation in the RbMHC class I α1 region makes it uncertain whether the molecule belongs to the non-classical group. However, because transcription was induced by megalocytivirus, the molecule is most likely a member of the classical group. Our results provide important information for future studies on antigen presentation and cytotoxic CD8+ T cell responses in rock bream against infectious pathogens.
収録刊行物
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- 魚病研究
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魚病研究 49 (3), 93-112, 2014
日本魚病学会
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詳細情報 詳細情報について
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- CRID
- 1390282681438491392
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- NII論文ID
- 130004785811
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- NII書誌ID
- AN00063165
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- ISSN
- 18817335
- 0388788X
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- NDL書誌ID
- 025842420
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可