マボヤ被嚢軟化症のPCR診断法の開発

DOI 日本農学文献記事索引 Web Site 被引用文献2件 参考文献9件 オープンアクセス
  • Kumagai Akira
    Miyagi Prefecture Fisheries Technology Institute, Freshwater Fisheries Experimental Station
  • Kamaishi Takashi
    National Research Institute of Aquaculture, Fisheries Research Agency

書誌事項

タイトル別名
  • Development of Polymerase Chain Reaction Assays for Detection of the Kinetoplastid <i>Azumiobodo hoyamushi</i>, the Causative Agent for Soft Tunic Syndrome in the Ascidian <i>Halocynthia roretzi</i>
  • Development of Polymerase Chain Reaction Assays for Detection of the Kinetoplastid Azumiobodo hoyamushi, the Causative Agent for Soft Tunic Syndrome in the Ascidian Halocynthia roretzi
公開日
2013
資源種別
journal article
DOI
  • 10.3147/jsfp.48.42
公開者
日本魚病学会

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説明

PCR assays were developed for the rapid and sensitive detection of Azumiobodo hoyamushi, the protistan pathogen that causes soft tunic syndrome in the cultured ascidian Halocynthia roretzi. Two sets of A. hoyamushi-specific primers based on the 18S rRNA and β-tubulin gene sequences, respectively, of the flagellate were designed and validated for the detection of A. hoyamushi. In both PCR assays, the flagellate genes were detected in diseased ascidians, but not in apparently healthy ones. When DNA were extracted from flagellate suspensions obtained from infected tunics, the detection limits of the PCR assays using primers for the 18S rRNA and β-tubulin were 3.4 × 10 cells/mL and 3.4 × 102 cells/mL, respectively. Even at the early stage of infection when the slight clinical signs were observed only in the siphons, A. hoyamushi was detected from the affected tissue using 18S rRNA PCR, but not β-tubulin PCR. Thus, the 18S rRNA PCR is a rapid, sensitive and specific assay for diagnosing soft tunic syndrome.

収録刊行物

  • 魚病研究

    魚病研究 48 (2), 42-47, 2013

    日本魚病学会

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