魚類寄生微胞子虫を検出するための迅速in　situハイブリダイゼーション法

DOI Web Site 被引用文献1件 参考文献6件
  • 李 宜〓
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • 横山 博
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • 小川 和夫
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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タイトル別名
  • Rapid In Situ Hybridization Technique for the Detection of Fish Microsporidian Parasites

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抄録

Rapid in situ hybridization (ISH) technique was developed for the detection of fish microsporidian parasites. All developmental stages of Glugea plecoglossi including the initial stage immediately after entry into the fish tissue could be detected by rapid ISH using DIG-labelled oligonucleotide probes. However, ISH signals were only faintly visible from mature spores of Microsporidium seriolae, while the pre-sporogonic stages were clearly positive.Thus, it is recommended to use both rapid ISH and Uvitex 2B stain for the detection of all stages of M. seriolae. The rapid ISH allows the whole process to be completed within 2h, and thus this technique is both convenient and practical as a diagnostic and research tool for studying the life cycle of microsporidians.

収録刊行物

  • 魚病研究

    魚病研究 38 (3), 117-119, 2003

    日本魚病学会

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