Three Cell Lines Derived from Spleen and Kidney of Black Porgy (Acanthopagrus schlegeli).

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  • Tung L.-C.
    Department of Zoology, National Taiwan University On Leave from Department of Biology, National Taiwan Normal University
  • Chen S.-N.
    Department of Zoology, National Taiwan University
  • Kou G.-H.
    Department of Zoology, National Taiwan University

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Other Title
  • クロダイの脾臓と腎臓から作出した3種の細胞系
  • クロダイの脾臓と腎臓から作出した3種の細胞系〔英文〕
  • クロダイ ノ ヒゾウ ト ジンゾウ カラ サクシュツシタ 3シュ ノ サイボウ

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Description

Three cell lines derived from spleen and kidney of black porgy (Acanthopagrus schlegeli), designated as BPS-1, BPS-4, and BPK, were established and their characteristics were determined. The BPS-1cell line and BPS-4 cell line consist of epithelial-like and fibroblast-like cells, respectively. The BPK cell line contains both fibroblast-like and epithelial-like cells. Using Leibovitz's-15 medium supplemented with 10%fetal calf serum and 0.15M NaCl at 28°C, BPS-1, BPS-4 and BPK cells have been passaged 105, 104, and 87 times, respectively.<BR>The chromosome numbers of the BPS-4 cell ranged from 10 to 58 with a modal number of 30 at passage 77, while the chromosome numbers for the BPS-1 cells and the BPK cells varied even more widely from 25 to 98 at passage 76 and 9 to 94 at passage 28, respectively.With low seeding densities, all the three cell lines showed a low plating efficiency.All three cell lines were susceptible to a variety of fish birnaviruses and herpesviruses from eels in Taiwan.

Journal

  • Fish Pathology

    Fish Pathology 26 (3), 109-117, 1991

    The Japanese Society of Fish Pathology

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