粘液胞子虫の感染ステージ（放線胞子虫）の採集方法とキンギョへの実験感染

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  • 横山 博
    Department of Fisheries, Faculty of Agriculture The University of Tokyo
  • 小川 和夫
    Department of Fisheries, Faculty of Agriculture The University of Tokyo
  • 若林 久嗣
    Department of Fisheries, Faculty of Agriculture The University of Tokyo

書誌事項

タイトル別名
  • A New Collection Method of Actinosporeans-A Probable Infective Stage of Myxosporeans to Fishes-from Tubificids and Experimental Infection of Goldfish with the Actinosporean, Raabeia sp.
  • 粘液胞子虫の感染ステージ(放線胞子虫)の採集方法とキンギョへの実験感染〔英文〕
  • ネンエキ ホウシチュウ ノ カンセン ステージ ホウセン ホウシチュウ ノ サ

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説明

We tried several transmission experiments of naturally infected actinosporeans in the tubificid oligochaete, Branchiura sowerbyi, to myxosporean-free goldfish, Carassius auratus.<BR>Almost all actinosporean spores collected by squashing tubificids or filtering effluent from a container with tubificids were physically damaged and/or unclean, and all infection experiments using such spores failed. However, coexistence of uninfected goldfish with a stock of B. sowerbyi shedding spores of several actinosporeans in the same container resulted in infections with three species of myxosporeans : Zschokkella, Myxobolus, and Thelohanellus spores in 15-20% of goldfish after 2.5-4 months. We improved the collection method of actinosporeans; tubificids were individually placed in the cell-well plates with 24 2 ml-wells and waterborne actinosporeans were detected by inverted phase-contrast microscopy. Actinosporeans harvested by this method were intact and purely concentrated. Eventually, in goldfish kept with tubificids harbouring the actinosporean Raabeia sp. in the same tank for 3 weeks, and in goldfish exposed to Raabeia suspension for 1 hour, spores of an unidentified Myxobolus were found 3-4 months after the exposure. Unexposed goldfish and ones exposed to actinosporean-free tubificids were both negative for the infection.<BR>We developed the collection method of actinosporeans, and confirmed that the actinosporean Raabeia infecting the oligochaete B. sowerbyi was transformed into Myxobolus in goldfish.

収録刊行物

  • 魚病研究

    魚病研究 26 (3), 133-138, 1991

    日本魚病学会

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